ZC2_5_metag - SRA

SRX18473288: ZC2_5_metag
1 ILLUMINA (Illumina NovaSeq 6000) run: 27.8M spots, 8.4G bases, 2.6Gb downloads

Design: Sediment samples (6 ml) were spiked with 700 μl of an overnight culture of Lactobacillus reuteri ATCC 23272 (Nanodrop-based OD600 = 0.28; representing 1.70 × 108 cells ml-1). DNA was extracted from the sediment samples using the RNeasy PowerSoil Total RNA kit and the RNeasy PowerSoil DNA Elution kit (QIAGEN, Toronto, Canada). The DNA extracts were treated with RNAse If (New England Biolabs, Whitby, Canada) and purified using Macherey-Nagel NucleoMag NGS beads (D-MARK Biosciences; Toronto, Canada). The purified DNA was quantified using the Quant-iT PicoGreen assay (Fisher Scientific Ltd., Edmonton, Canada). Metagenomic sequencing libraries were prepared with 1 ng of DNA using the Nextera XT DNA Library Preparation Kit from Illumina (San Diego, USA). Equimolar amounts of the prepared libraries were pooled and sequenced on an Illumina NovaSeq 6000 S4 PE150 system in a 2x150bp configuration.

Submitted by: National Research Council Canada

Study: Microbial communities of beaches of the Baffin Island Oil Spill (BIOS) Project almost 40 years later

PRJNA906105 • SRP411080 • All experiments • All runs

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This project re-visited beaches of the 1980s Baffin Island Oil Spill (BIOS) Project in 2019, almost 40 years after the original project. Sediment samples were collected from both the previously oil-exposed beach test plots and nearby control sites to characterize the corresponding microbial communities. The goals of this project were to: (i) assess any long-term legacy effects of the 1980s oil exposure, (ii) compare the microbial communities between the different oil-exposed beach sites, and (iii) identify potential oil-degrading microorganisms that may have been enriched since the original oil exposure.

Sample: Bay 106, plot IMEc, 0.05m, replicate B

SAMN31975109 • SRS15947019 • All experiments • All runs

Organism: beach sand metagenome

Library:

Name: ZC2_5_metag

Instrument: Illumina NovaSeq 6000

Strategy: WGS

Source: METAGENOMIC

Selection: Hybrid Selection

Layout: PAIRED

Runs: 1 run, 27.8M spots, 8.4G bases, 2.6Gb

Run	# of Spots	# of Bases	Size	Published
SRR22508338	27,806,392	8.4G	2.6Gb	2022-12-02

ID:: 25511114

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